abcam特约代理商|ab6870 羊抗人IgG H&L (

手把手课程之 IHC 关键操作步骤

)Secondary Antibody15mlIHC-P8125SSignalStain® Boost IHC Detection Reagent (HRP, Mouse)Secondary Antibody15mlIHC-P8889SAnti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate)Secondary Antibody250µlIF-IC F8890SAnti-mouse IgG (H+L), F(ab

T-cell dependent antigen specific in situ staining (Pe)

endogenous peroxidase by incubating in TBS-T 0.1%NaN 3 and 0.3% H 2 O 2 for 30 min.  Wash thrice. Top of Page             Choose next steps: HRP-conjugated secondary Ab Tyramide amplification Biotin-conjugated secondary Ab

细胞凋亡如何检测？

H2O 1.84 g 柠檬酸 0.51 g DDW 100 ml 6. 显色液(现配现用)：底物Buffer 10 ml OPD 2 mg 30% H2O2 2 ml 7. 终止液 2 Mol/L H2SO4 8. 重组人TFAR19, HRP标记的羊抗人IgG9. ELISA板, Tip, 移液器，ELISA Reader(OD490 nm)，洗板机 操作步骤 1. 用包被Buffer稀释的重组人TFAR19(1 mg/ml)包被ELISA板, 100 ml/well, 37