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上海玉博生物科技有限公司
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文献和实验Efficient Transformation of Yeast - LiAc Method
xTE, 1ml 10xLiAc, LiAc sol. 1ml 10xLiAc, 1ml 10xTE, 8ml H2 O (water) Inoculate cells from a single colony into 20ml YPDA and incubate at 30°C until OD600 =1-2. Transfer enough of this culture to a 300ml YPDA medium
SMALL Scale Transformation 1. Inoculate 1 ml of YPDA or SD with several 2�3 mm colonies. 2. Vortex vigorously to disperse any clumps. 3. Transfer cells to a flask containing 50 ml of YPDA or SD: 4. Incubate
Preparation of Yeast Protein Extracts
selection medium (SD-Leu or SD-Trp). 2. Also set up untransformed yeast as a control. Leave at 30 ° C, 230-250rpm, for 18-24h. 3. For each clone to be assayed, separately inoculate 50ml of YPDA with the entire O/N culture. Take an OD
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