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文献和实验Green Fluorescent Protein (GFP) as an Indicator of Transfection in Chicken Embryos
/negative genes can be introduced to study the effect of signal interference on cells. This basic protocol is concerned with the application of GFP as an indicator of transfection in chicken embryos. Lipofectin® Reagent (Life Technologies, catalog
Method for Single-Cell Electroporation
high transfection rates, and requires relatively inexpensive, common laboratory equipment. A paper of this technique is published in the March issue of Neuron, 2001. This technique utilizes electroporation in which a brief external high voltage
. Lissandron, V., Terrin, A., Collini, M., D'alfonso, L., Chirico, G., Pantano, S., and Zaccolo, M. 2005. Improvement of a FRET‐based indicator for cAMP by linker design and stabilization of donor‐acceptor
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