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文献和实验MitoProbe DiIC1 Mitochondrial Membrane Potential Protocol
to changes in membrane potential. 1. Labeling Cells with DiIC1(5) Before beginning the experiment, ensure that the vials of DiIC1(5) and CCCP have equilibrated to room temperature. 1) For each sample, suspend cells in 1 mL warm medium
Synthesis and Probing of Membrane-bound Peptide Arrays
from the nitrocellulose membrane by gently placing tissue onto it. To avoid damage to the adsorbed protein, do not wipe or press tissue onto the membrane. Detect the spots using a chemiluminescence detection kit (e.g., ECL
Random Primer Labeling Kit (Stratagene,300385) [alpha-32P]dCTP (ICN) Salmon Sperm DNA (Stratagene,201190) SDS solution,20% w/v (Bio-Rad,161-0418) Zeta-Probe Genomic Tested Blotting Membrane (nylon)(Bio-Rad,162-0196) Gel Blot Paper GB002 (Schleicher
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