SBI特约代理商|EXOGR800A-1 ExoGlowTM

Protocol for 5' End Labeling RNA

of the individual components of the kinase reaction, below.   2. Protocol for 5' End Labeling RNA 1) Combine the following in a single RNase-free microfuge tube: -- µl nuclease-free water (to make a final volume of 20 µl) -- µl RNA

Total Bacterial RNA Labeling with Random Hexamers.

  Total Bacterial RNA Labeling with Random Hexamers. Arkady Khodursky, Ph.D. Email: khodursk@cmgm.stanford.edu Updated: 2/10/99 20-25ug of total RNA in 10-14 ul of diH20 should be purified using RNA kit or hot phenol extraction

Genomic DNA Labeling Protocol

(as part of the BioPrime labeling kit), produces better labeling. 6. Incubate 37 degrees C for 1 to 2 hours, then stop reaction by adding 5 ul 0.5 M EDTA pH8.0   7. As with RNA probes, I purify the DNA probe using a microcon 30 filter (Amicon/Millipore