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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
Methods for the Detection of D-Amino-Acid Oxidase
al. ( 5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
v468 Chapter 6 细胞内结肠腺瘤样息肉蛋白核定位以及细胞质检测
cancermutations (12) . These techniques are outlined in this chapter. 2. Materials 2.1. Cell Culture 1. Human SW480 (APC truncated at amino acid 1,338), HT-29 (APC truncated at amino acids 853 and 1,555), andHCT 116 (full-length APC) colon
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