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文献和实验transfer vessels. Each day students will observe these transfer cultures with an inverted phase contrast microscope, and remove aliquots for cell counting with a hemacytometer. Simultaneously, they will check on the viability of the cells through a dye
Purpose Working Medium 2) Cell viability and counting a. Pipet 250 μL of a 0.05% Trypan blue solution into one, 1 mL polystyrene round-bottom tube. b. Homogenize the HepaRG™ cell suspension with gentle manual swirling
Endothelial Cell Systems - Instructions For Use
-5022)1 x 100 ml bottle Trypsin/EDTA Solution (CC-5012) 1 x 100 ml bottle Trypsin Neutralizing Solution (CC-5002) 1 x 100 ml bottle NOTE: If you use a different Clonetics® medium, see Appendix A, Endothelial Cell Media Endothelial Cell Systems Media
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