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文献和实验Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
Two dimensional peptide mapping
from the protease adsorbing onto the filter. 3. Aspirate the liquid. Wash the membrane extensively with H2O (5 X 1ml). Then wash with freshly-made 0.05 M NH4HCO3 once or twice. 4. Digestion: Add 150 -200 microliter fresh 0.05 M NH4HCO3
Synthesis and Probing of Membrane-bound Peptide Arrays
-transfer the proteins bound to the peptide spot membranes onto the nitrocellulose membrane for 1 hr at 0.85 mA/cm2. IMPORTANT: Due to SDS denaturation, all proteins will be negatively charged. Therefore, the nitrocellulose should be placed
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