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- 2025年07月15日
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文献和实验. 2.1.3. What are acceptable enzyme diluents for Deoxyribonuclease (D/DPFF/DPRF)? D/DPFF: May be dissolved in 5mM acetate, 1 mM calcium, pH 4.5 and stored in single use aliquots at -20 or -70 for 1 yr. DPRF: Use water or any buffer pH
Preparation of High Titer Adenovirus in P11 cells
deoxyribonuclease I (Dnase I) in 10 ml of 10 mM Tris-HCL, pH 7.4, 50 mM Na Cl, 1 mM DTT, 0.1 mg/ml BSA and 50% glycerol. Store in small aliquots at �20° Use sterile TE for saturated CsCl solution and store at 4° , but equilibrate to RT prior to use.
Appendix: How to prepare the DNaseI column. Variant 1. Coupling DNase I to CNBr-Sepharose 1.Deoxyribonuclease I (Worthington biochemical corporation, Halls Mill Rd. Freehold, New Jersey.07728) 54B237, 100 mg, $211. Dissolved in 10 ml
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