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文献和实验Figure 5.13.2 Preparation of 6 . (a) 1,2,4‐triazole, TEA, POCl3 , MeCN:DCM (1:1 v/v); (b) sodium salt of 1,7‐heptanediol, dioxane; (c) Pac‐Cl, TEA, THF; (d) TBAF (1 M in THF), THF; (e) Cl‐P(OCE)N i Pr2 , DIPEA
Quantitation of Protein:Bicinchoninic Acid (BCA) Method
of amino acids can bind with Cu 2+ to form a complex in the basic solution and then the Cu 2+ is deoxidized to Cu + . Bicinchoninic acid and its sodium salt are water-soluble. In the alkaline environment, it can bind with Cu
In Situ Hybridization Using Digoxigenin Labeled Probes
on two 5ml pipettes in a tray lined with moist paper towels. Apply 100µl of a 1:500 dilution of Anti Digoxigenin-POD in 1X PBS, 5mMEDTA. Distribute the solution by placing a 22 x 40 mm coverslip on the slide. 5) Cover the tray with plastic wrap
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