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文献和实验TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
Fisherbrand SuperFrost/Plus positively-charged microscope slides (Cat. No. 12-550-15) for all of our frozen tissue sectioning and have very good tissue retention on slides after an in situ hybridization experiment. SuperFrost/Plus slides require no preparation
TISSUE PREPARATION FOR IN SITU HYBRIDIZATION
/PLUS MICROSCOPE SLIDES FOR IN SITU HYBRIDIZATION We use Fisherbrand SuperFrost/Plus positively-charged microscope slides (Cat. No. 12-550-15) for all of our frozen tissue sectioning and have very good tissue retention on slides after an in situ hybridization
the RNase 1:2000 with PBS to give a working concentration of 50 µg/mL. 11) Microscope slides � Fisherbrand Superfrost slides (#12-550-14). Coat slides with a 1:10 dilution of poly-l-lysine (Sigma #P8920) in distilled water. Differential
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