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上海玉博生物科技有限公司
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文献和实验GE Healthcare Benzamidine Sepharose™ 6B is p-aminobenzamidine covalently attached to Sepharose 6B by the epoxy coupling method. p-Aminobenzamidine (PAB), is a synthetic inhibitor of trypsin-like serine protease. Trypsin and trypsin
, and 20 mMTris-HCl, pH 7.5, sterile filtered. 5. Syringe (30–50 mL) with 0.2-μm pore size filter. 2.3.2. Step 2: IMAC 1. Sample: Pooled Wnt3A containing fractions from Section2.3.1 . 2. HiTrap? Chelating, 1-mL column (GE Healthcare, Piscataway
and Superdex 200p.g.) were packed into XK 16/40 or Tricorn 10/30 columns. AEC media were packed into Tricorn 10/15 columns. First experiments with ion-exchangers were conducted with HiTrap screening columns (all from GE Healthcare Bio-Sciences, Sweden). Void
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