Click Chemistry Tools特约代理商|112

Synthesis of Alkyne‐ and Azide‐Modified Oligonucleotides and Their Cyclization by the CuAAC (Click) Reaction

min. The resultant duplexes have very high thermal stability and their CD spectra are characteristic of normal B?DNA. Curr. Protoc. Nucleic Acid Chem. 35:4.33.1?4.33.21. © 2008 by John Wiley & Sons, Inc. Keywords: Click chemistry

Coupling Peptides To Resin For Affinity Purification

checking the resin chemistry using a quick eyeball test. The amino resin will react with an NHS ester, whereas the original affigel and the iodoacetate will not. Take an aliquot (50ul) of resin at each step. Resuspend in 100 ul of buffer. Add 1 ul of 0.1 M

Coupling Peptides To Resin For Affinity Purification

volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate for 10 min at RT. The resin has about 10 5mol groups/ml, so add