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文献和实验Chromatin Immunoprecipitation (ChIP)
gel Antibodies to native protein of interest (or a post-translationally modified form) Antibody (control) Cell lysis buffer for ChIP (cold) Cells (50-100 million per experiment) Dilution buffer EDTA Elution
Herring Sperm DNA37% Formaldehyde (ACS reagent grade)1.25 M glycineProtocol: (Generalized for all cell types- inquire for details regarding particular cell types) For all the following steps,use the pipets that are specifically designated for ChIP use
CHROMATIN IMMUNOPRECIPITATION (CHIP) PROTOCOL FOR YEAST This protocol is derived from a paper by Miriam Braunstein and is based on work in the Allis lab. The procedure was written by Pam Meluh and updated by Paul Megee (6/10/00).
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