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上海玉博生物科技有限公司
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文献和实验-cryopreserved) Apheresis Cells 1) Dilute PBMC to 5-10 x 106 cells/ml in Incomplete Medium (pre-warmed to 37°C). 2) Wash Dynabeads® ClinExVivo™ Epoxy as described above for Dynabeads® CD3⁄CD28 CTS™. 3) Add Dynabeads®
Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
. Note: CELLstart™ CTS™ substrate may be substituted for Geltrex® hESC-Qualified Matrix 3. Thawing and Plating hESCs 1) Label the Geltrex® matrix-coated dish with the passage number from the vial, the date, and user initials. 2)
Procedure for Culturing BG01V Human Embryonic Stem Cells with Human Feeder Cell Conditioned Medium
Conditioned Medium to 37° C. Thaw the vial of BG01V hES cells by warming until just thawed and then immediately transfer to a 15 mL conical tube containing at least 5 mL of pre-warmed Human Feeder Cell Conditioned Medium. Spin
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