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Purification of Human Multiprotein Complexes using OneSTrEP Technology

. For more theoretical background and a protocol for purification of recombinant Strep -tag® fusion proteins from E.coli please refer to Schmidt and Skerra (2007). In comparison to other commonly used purification strategies (i.e. HA and FLAG double-tag

Introduction of human 14-3-3 proteins

of mRNA encoding human 14-3-3 protein,a protein kinase-dependent activator of tyrosine and tryptophan hydroxylases and an endogenous inhibitor of protein kinase C.The 1,730-nucleotide sequence of the cloned cDNA contains 191 bp of a 5'-noncoding region

PriCells: Methods of Isolation and Culture of Human Hepatocytes

PriCells: Methods of Isolation and Culture of Human Hepatocytes Safety note. When working with human material, a solution of medical disinfectant, i.e., Virkon solution (2%), should always be available to disinfect spills. All waste media