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上海玉博生物科技有限公司
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文献和实验(1,4-diazabicyclo(2.2.2.) octane) Sigma D2522 800 µl ddH2O 200 µl 1M Tris-HCL, pH 8.0 9 ml glycerol Keep at 4 C covered in foil Add 30 µl/slide and use large coverslip Store slides at 4 C in the dark 上一篇:Whole mount
protein concentration: protein (mg/mL) = (A280- (A492 x 0.35))/1.4 d) Calculate moles of protein: = protein(mg/mL)/1.5x10^5 e) Calculate moles of FITC: = A492/0.69x10^5 f) Determine F/P ratio: = moles of FITC/moles of protein g) F/P ratio
monoclonal antibody production fusion
), 3 L, 0.22 (or 0.45) µm-filtered, store at -20ѓC = Asp-Arg x100, 1 L + Gluta x 50, 2 L + mercaptoethanol, 350 µl Asp-Arg x100, 1 L, pH 6.0 = L-Asparagine, 3.6 g (0.24 mM) + L-Arginine (0.55 mM), 11.6 g + HCl, 0.5N, 100 ml -> heat to 37ѓC
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