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文献和实验cDNA Library Construction from Single Cells
expression. J. Neurosci. Methods 77:43‐48. Crino, P.B. and Eberwine, J. 1996a. Molecular characterization of the dendritic growth cone: Regulated mRNA transport and local protein
Analysis of mRNA Populations from Single Live and Fixed Cells of the Central Nervous System
a method for the amplification of poly(A)+ mRNA extracted from the cytoplasm of a single cell. After cDNA is synthesized from the mRNA, it is made double stranded, denatured, and reverse transcribed to yield antisense RNA (aRNA
Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
Outline: Bacteriophage lambda is a linear double stranded DNA, approximately 50 kB in size. The two sticky ends help the phage to recircularize after entering the bacterial host cell. A cDNA library is a representation of mRNA's
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