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文献和实验Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
should be shake gently by rotating blood mixer (vortex) Pour 500 µl of blood into a 1.5 ml eppendorf tube and add 1000 µl of red cell lysis buffer. Shake microfuge tube gently (up to homogenizing), then spin for 2 minutes at 7000 rpm. Discard
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood
gradually with the freezing chamber. Procedure: Thawing cells for transformation: Freezing cells: Pipette 1.0 ml of whole blood into 2 cryotubes (1.25ml). Add to each cryotube 100
Human Peripheral Blood Mononuclear Cell Preparation
and institutional policies for proper handling instructions for human blood.2. The bubbles trap red blood cells.3. To deplete monocytes, culture the mononuclear cell mixture in several 10 cm dishes for 30 minutes to 1 hour. Monocytes adhere to the plastic dish
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