pierce特约代理商|28005 Pierce™ Biot

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

of choice (15µg, Lipofectamine 2000™ Invitrogen). The following day the flag-tagged protein expressing plasmid transfected cultures are transfected again with 100nM EF52 biotin labeled siRNA (antisense or sense alone) using Lipofectamine 2000™ or stock 3.4µM

Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems

polymerase I (unit 3.5 ) TE buffer ( appendix 22 ) Biotin‐cellulose (Pierce

Introduction to the EMSA (Gel Shift) Technique

Chemiluminescent EMSA Kit, Product # 20148 ) and a kit to facilitate labeling DNA with biotin (Biotin 3´ End DNA Labeling Kit, Product # 89818 ). The LightShift EMSA Kit utilizes Pierce’s patented SuperSignal Chemiluminescent Detection Technology to offer detection