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文献和实验Lentiviral Vector Mediated Transgenesis
with the three?decade?old DNA pronuclear injection technique, lentivector?mediated transgenesis is simple, cheap, and highly efficient. Furthermore, it can take full advantage of the great diversity of lentiviral vectors developed for other applications, and thus allows
Creation and Use of Infectious Virus Vector
Creation and use of your infectious vector: Plate 5 x 105 293T cells in 6 cm2 dishes containing 5 ml of media. (This can be scaled up if desired). The following day set up (use polypropylene tubes
Protein Chip for Detection of DNA Mutations
gene to the vector pET32a (+) sequentially with insertion of a (Ser-Gly)6 coding sequence before and behind Strep tagII gene, respectively. The fusion protein THLSLM was expressed in Escherichia coli AD494 (DE3) and purified using Ni2+ -chelation
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