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上海玉博生物科技有限公司
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文献和实验Elmer) Random hexamer primers (Perkin Elmer) MMLV reverse transcriptase, 100 units/µl (GenHunter) 10X PCR buffer (Perkin Elmer) AmpliTaq DNA polymerase (Perkin Elmer) DEPC- treated H2O 3 M Na acetate, pH 5.2 (Life
细胞/组织中的DNA定量(Quantification of DNA in cell / tissue samples)
・ Standard range assay solution (DNA samples: 10 ng/well- 500 ng/well)0.1 µg/ml Hoechst 33258Dye stock 10 µlDPBS 100 mlPrepare fresh daily ・ Extended range assay solution (DNA samples: 100 ng/well � 4000 ng/well) 1 mg/ml Hoechst 33258 Dye stock
the 1 ul sample for A and C reactions, and the 2 ul sample for G and T reactions. Meanwhile, preheat the PE Cetus Thermocycler 9600 to 95degC (Program #2). 2. Prepare the Taq polymerase dilution. AmpliTaq polymerase (N801-0060) is from Perkin-Elmer Cetus
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