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文献和实验. SNPs were assorted into multiplex groups and constituent PCR primer and probes were designed using SpectroDESIGNER software version. 2.0 (Sequenom, San Diego, CA). The assay design filter for SNP sequences is 2 alleles. The length of the amplicon (Bp
), Molecular Beacons, Scorpions® and SYBR® Green (Molecular Probes), are available for real-time PCR. All of these chemistries allow detection of PCR products via the generation of a fluorescent signal. TaqMan probes, Molecular Beacons and Scorpions depend on F
increase in fluorescence is observed. In contrast, an endpoint assay (also called a “plate read assay”) measures the amount of accumulated PCR product at the end of the PCR cycle. About Sequence Detection Chemistries Overview Applied Biosystems
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