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上海玉博生物科技有限公司
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文献和实验Method for Single-Cell Electroporation
of neurons in the tadpole brain was high with exponential decay pulses with peak voltages of 20 V and t = 70 ms. Slightly higher transfection efficiency was achieved with 0.5-1 s trains of 1 ms long square pulses at 50 V and 200 Hz. We found that 5 repeated
purification of protein complexes, in combination with in vivo biotinylation of critical transcription factors, has contributed to the analysis of the pluripotent state in mouse embryonic stem (ES) cells and made it possible to construct a protein?protein
Construction and Characterization of Adenovirus Vectors
, dilute the 1X stock solution with H2 O and then sterilize by autoclaving for 45 min at 121°C. Transfection reagent (e.g., SuperFect; QIAGEN) Trypsin-EDTA (10X) (Invitrogen 15400054) Dilute the 10X stock solution with PBS-Ad to 1X
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