- ￥200000
- Polyplus Transfection
- 201-50g
- 2025年07月14日
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上海玉博生物科技有限公司
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文献和实验Transient transfection into 293T cells
: optional) • Transfection reagents: i) 2M CaCl2 : 2M in H2 O, filter sterile, store at 4°C ii) x2HBS: 8.0g NaCl, 0.37g KCl, 201mg (yes! mg) Na2 HPO4 •7H2 O, 1.0g glucose, 5.0g HEPES/500ml (adjust pH to 7.05 with NaOH and filter sterile
Ultrasound-Mediated Gene Transfection
) setups. Although high transfection rates have been attained in some of these setups in vitro, replicating similar levels of transfection in vivo has been difficult. In vivo-simulated setups offer hope for a more consistent outcome in vivo. Presented
Preparing Vector DNA For Transfection/Targeting
if need be). -To each 1/2 of a prep, containing approximately 200-250 µg of supercoiled DNA, add the following: 40 µl of 10 x Enzyme buffer 50-100 units enzyme (I prefer a new tube of enzyme for this) bring up to 400 µl with sterile ddH2O. Digest
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