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上海玉博生物科技有限公司
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文献和实验CHAPS Insoluble Floating Fraction Preparation
7.5, 50 mM NaCl, 2 mM EDTA). They were finally resuspended in TEBP (TEB plus various protease inhibitors: 1x PIC I and 1x PIC II) to 2 x 108 /ml. CHAPS powder was added at 20 mg/ml. After gentle mixing, the lysed cells were kept on ice for 3-5
Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
. After the mortars are cooled, nestle them in the dry ice.Collect 1 g of tissue and flash freeze in liquid nitrogen.Transfer to a cooled mortar and pestle. Grind tissue in liquid nitrogen until it is a fine powder.Remove one pre-warmed glass homogenizer. Add 8 mL
in 10% HCl) Add 0.1 grams of FeCl to 50 ml of 10% (v/v) HCl and make to 100 ml with 10% HCl. Acrylamide Solutions Acrylamide solutions for PAGE are given as total
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