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文献和实验Carbohydrate‐Oligonucleotide Conjugates
Bouillon, C., Meyer, A., Vidal, S., Jochum, A., Chevolot, Y., Cloarec, J.P., Praly, J.P., Vasseur, J.J., and Morvan, F. 2006. Microwave assisted “click” chemistry for the synthesis of multiple labeled‐carbohydrate oligonucleotides on solid support. J. Org
Replication timing by density transfer
-Aldrich (previously from Isotec, which is now part of Sigma-Aldrich) "-N" medium =1.61 g yeast nitrogen base w/o amino acids and ammonium sulfate, 11.1 g succinic acid, 6.67 g NaOH per litre. Pronase : We use
Yeast Media, Solutions and Stocks
Yeast Media: Medium using 6.7 g yeast nitrogen base without amino acids (Difco # 0919-15) can also be prepared using 1.7 g yeast nitrogen base without amino acids and ammonium sulfate (Difco #0335-15) and 5.0 g ammonium sulfate
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