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文献和实验Immunohistochemistry (IHC) of Thymus Frozen Sections
a slide from PBS and wipe edges of each slide with KimWipe to create a static barrier. Add 175 µL of 1º antibody to each slide. Cover with a lid x1hr. Rinse by dipping in 2 staing jars containing PBS. Soak in a 3rd PBS jar x5 min. Add 175
(against the third target protein) followed by detection using a mixture of Streptavidin NorthernLights™ NL-493 tag (green fluorescence) and anti-DIG secondary antibody conjugated to a Rhodamine Red X™ tag (red fluorescence). This technique provides good spectral separation
the 3 hour incubation Dynabeads™ M-280 Streptaviden magnetic beads (7x107 beads, ~100µl/sample) are added to the respective samples (note 1);5.The resultant siRNA/Flag complexes are then eluted with magnetic bead binding, the solution can be discarded
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