roche 代理商|11277073910 DIG RNA

Digoxigenin-UTP RNA labeling

1. Add the following to a sterile microfuge tube on ice: -1 ug linearized plasmid DNA. -2 ul Dig RNA Labeling Mix, 10X concentrate -2 ul 10X transcription buffer -1-2 ul RNase inhibitor (DNase free) -add DEPC treated H2O up to 18 ul -2 ul RNA

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, or Fluorochromes Procedures for Labeling DNA, RNA, and Oligonucleotides with DIG, Biotin, or Fluorochromes 41 Random primed labeling of ds DNA with DIG-, Biotin- or Fluorescein-High Prime reaction mix 41 PCR labeling of ds DNA with the PCR DIG Probe Synthesis Kit

地高辛（DIG）产品选择指南——选择合适的标记

  如果您需要制备RNA探针的话，推荐您使用Northern Starter Kit。该试剂盒以线性的DNA为模板，通过SP6, T7或是T3 RNA聚合酶的作用将DIG-11-UTP掺入到转录的RNA中，借助化学发光底物CDP-Star检测。另一个产品DIG RNA Labeling Kit也可用于RNA探针的标记。   末端标记寡核苷酸Oligonucleotide