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文献和实验a very good article,hope to study together with you!:) ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION INTRODUCTION Immunoprecipitation is a procedure by which peptides or proteins that react specifically with an antibody are removed
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
with an antibody are removed from solution and examined for quantity or physical characteristics (molecular weight, isoelectric point, etc.). As usually practiced, the name of the procedure is a misnomer since removal of the antigen from solution does not depend
for primary antibody), labeled with alkaline phosphatase or horseradish peroxidase. Substrate appropriate to the enzyme conjugate (HRP or AP). Appropriate wash buffer: Phosphate-buffered saline (PBST): 10 mM sodium phosphate, pH 7.2, 0.9% (w/v
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