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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
-prep RNA probed with ccg-1 DNA fragment. Total RNA from a series of transformants into bd A and frq7 A was examined for the presence of the ccg-1 gene transcript (Loros et al. 1989 Science 243:385-388). Each lane contains 10 µg of RNA (1/200
Mag-Bind Plant RNA Protocol using 96-well Plate
16. Remove plate from magnet and add 48uL DNase I Digestion Buffer and 1 uL DNase I. Resuspend the magnetic beads by pipetting or vortexing. Note: If total nucleic acid (both DNA and RNA) are desired, skip the DNase I digestion step
Extraction of DNA using DNAzol® Reagent
pipette tips by cutting 2-3 mm from the ends of plastic pipette tips. Mix DNA solutions by inversion; avoid shaking or use of a Vortex for mixing. Homogenization of tissues: Homogenize tissue samples in a hand-held glass/Teflon® homogenizer
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