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文献和实验Estimation of Fat in Milk Samples
Introduction Milk fat measurement is a common task in the dairy industry, because milk fat content is one factor that determines milk price and is necessary to know for casein/fat ratio normalization. It is also important
Blocking of unwanted non-specific staining
7.5, to which 0.01 Tween 20 has been added (TBS-T). 2- Briefly blot the slides without letting them dry and then apply egg white in PBS-BSA-NaN3 as a blocking agent (one egg white in 100 ml PBS + NaN3) (Miller RT et al, Appl Immunohistochemistry 5(1): 63-66
µl PlateBlock™ for 2 h 4. Aspirate or tap the plate (do not wash) 5. Stabilization with Liquid Plate Sealer® animal-free for 2 - 15 min 6. Aspirate or tap the plate (do not wash), dry and store 7. Dilute the patient sample (1:100) in LowCross
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