thermo特约代理商|89952 NAb™ Protein

Protein‐Protein Interactions Identified by Pull‐Down Experiments and Mass Spectrometry

, and spin columns. Alternative techniques are detailed for isolating activation state?dependent protein interactions with small GTPases. Appropriate methods of sample preparation for mass spectrometry?based identification of interacting proteins

Protein Microarrays for Identification of Novel Extracellular Protein‐Protein Interactions

5% milk in PBST (see recipe ) PBST (see recipe ) Low‐protein‐binding 0.2‐µm

Purification of KHC Motor Domain Protein

), then with HEM + 200 mM NaCl (8 x 1 mL fractions). The protein peak elutes with 200 mM NaCl in the 2nd to 5th fractions. 11. If further purification is necessary, reduce volume using a Centricon 30 spin column, then add 1 volume HEM