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文献和实验TaqMan Primer and Probe Design
Adapted from TaqMan® One-Step RT-PCR Master Mix Reagents Kit Instruction Design of Probes Keep the G-C content in the 20 to 80% range. Avoid runs of an identical nucleotide. This is especially true for guanine, where runs
实验原理 Dynabeads® CD3⁄CD28 CTS™ offer a simple method for isolation, activation and expansion of human T cells. Firstly, CD3 T cells are separated and concentrated from the apheresis product by magnetic cell separation
Screening Colonies by Hybridization with Radiolabeled Probe
. Stop the reaction by adding 2 µl of stop mix. Purify probe with either one of the following kits: NucTrap® Probe Purification Push Columns (Stratagene #400701), for DNA- or RNA-probes 17 bp - 50 kb Qiaquick PCR
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