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文献和实验Fastfilter Plasmid Midi Kit Spin Protocol
with a volume of a least 3-4 times the volume of the culture and dilute the starter culture 1/500 to 1/1000 into growth medium. Following overnight bacterial growth, an OD600 of 1.5~2.0 indicates a well-grown culture. For the best result determination of OD600 for each culture
Fastfilter Plasmid Maxi Kit Spin Protocol
with a volume of at least 3-4 times the volume of the culture and dilute the starter culture 1/500 to 1/1000 into growth medium. Following overnight bacterial growth, an OD600 of 1.5~2.0 indicates a well-grown culture. For the best result determination of OD600 for each culture
Native Chromatin Preparation and Illumina/Solexa Library Construction
(1X PBS containing 0.5 % bovine serum albumin, filtered) T4 DNA ligase (400 U/µL) and 10X buffer Taq polymerase (New England Biolabs) TaqMan PCR master mix (Applied Biosystems) (see Step 38) TE (1X, pH 7.4) Triton X-100
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