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文献和实验Chromatin Immunoprecipitation (ChIP)
, which has a higher affinity for some antibody isotypes and for antibodies from some species. Problem: High background is observed using the negative control antibodies. [Step 30] Solution: There are several possible causes and solutions
ARNT Knockout genotyping by PCR
from each reaction on a 1% agarose gel. Master Mix: 5ul 10x PCR buffer ( with MgCl2 1.5mM final) 4 ul dNTP mix (2.5 mM each) 1 ul OL855 (5 uM) 1 ul OL856 (5 uM) 0.5 ul Taq 34.5 ul distilled, deionized, sterile water 4 ul mouse tail DNA
require compensation controls where the same cells are stained with each of the fluorochromes separately. The correct negative control for allimmuno fluorescent experiments are cells treated in the same way but incubated with isotype matched control
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