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文献和实验-stranded DNA or RNA. RNase A is an endoribonuclease that specifically hydrolyzes RNA after C and U residues. Cleavage occurs between the 3'-phosphate group of a pyrimidine ribonucleotide and the 5'-hydroxyl of the adjacent nucleotide. The reaction
能够非常有效的抑制基因的表达。Figure 1. Silencing Gene Targets by RNase III Derived siRNA Cocktails. A 200 bp dsRNA (15 µg) for each gene of interest was digested with 2.5 U RNase III for 1 hour at 37℃. 1A. RNase III efficiently digests dsRNA . One microgram
Ribonuclease A (RNase A), DNase-free
FeatureThe RNase A is free of DNase activity. It is not necessary to heat it before use.DescriptionThe Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single-stranded RNA at C and U residues. It cleaves the phosphodiester
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