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PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID

to remove any debris.       The embryos are washed in KSOM medium and cultured in organ culture dish in KSOM at 37o C, 5% CO2. Production of tetraploid embryos Fusion of the blastomeres of 2-cell stage embryos occurs when a square pulse is applied

Cell  Isolation  Techniques

normally cease when confluence is reached. Harvesting cells for study, processing or subculture requires dissociation and detachment of the monolayer. Limited treatment of the cell layer with the enzyme trypsin is the method most frequently applied

Live-Cell Imaging Combined with Immunofluorescence, RNA, or DNA FISH to Study the Nuclear Dynamics and Expression of the X-Inactivation Center

ES cells, we generated cell lines containing TetO arrays in the X-inactivation center, the locus on the X chromosome regulating X-inactivation, which can be visualized upon expression of TetR fused to fluorescent proteins. We will use