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文献和实验PURIFICATION OF PCR PRODUCTS WITH SEPHAD
microtiter plate (Evergreen from Phenix, catalog #LMP-8001. Secure with rubber bands and spin them down at 750x g for 90 sec. Remove the microtiter plate (these can be re-used until they break) and replace it with a new, labeled MJ Research
PURIFICATION OF PCR PRODUCTS WITH SEPHADEX
of parafilm. Incubate for 4 hours at room temperature. After 4 hours, place it on top of a 96-well plate 500ul microtiter plate (Evergreen from Phenix, catalog #LMP-8001. Secure with rubber bands and spin them down at 750x g for 90 sec
Gene Knockout Techniques(1) The Knockout Process
volume each) in 384-well polypropylene PCR plates, using 2 ul of input DNA sampled from the column pool plates. Alternatively, you can do 25 ul reactions with 5 ul of input DNA in 96-well plates, but the number of possible genes screened for will be smaller
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