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Human Monoclonal Antibodies: The Residual Challenge of Antibody Immunogenicity

One of the major reasons for seeking human monoclonal antibodies has been to eliminate immunogenicity seen with rodent antibodies. Thus far, there has yet been no approach which absolutely abolishes that risk for cell-binding antibodies

Monoclonal Antibody Production

drip off the end of the tail until you have collected ~200 µl.(You may have to pre-bleed twice,with a week or so between bleeds). (5) Take the collected blood and place at 37 ℃ for 30 min.to remove complement. (6) Place blood at 4 ℃ overnight to clot

Monoclonal Antibody Production Protocol

/ml). Add Aminopterin (2ml to 200 ml of medium). (Some workers will leave the cells at this point for 24 hours before adding Aminopterin. We add the drug immediately.) Seed the cells in 96 well microtiter dishes, 250 ul per well, 8 plates per fusion