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文献和实验Monoclonal Antibody Production Protocol
/ml). Add Aminopterin (2ml to 200 ml of medium). (Some workers will leave the cells at this point for 24 hours before adding Aminopterin. We add the drug immediately.) Seed the cells in 96 well microtiter dishes, 250 ul per well, 8 plates per fusion
The production of monoclonal antibody by hybridoma
, A., Popkov, M., Cortez, X., Fuller, R., Elia, M., Kerwin, L., and C.F. Barbas III. (2003) Rapid monoclonal antibody generation via dendritic cell targeting in vivo. Hybridoma and Hybridomics 22 (1), 23-31.
/P ratio: = moles of FITC/moles of protein g) F/P ratio should be between 5:1 and 6:1 for Flow Cytometric use. 上一篇：Testing Rabbit Bleeds By Elisa 下一篇：monoclonal antibody production fusion
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