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文献和实验Peptide Inhibition ELISA Protocol
Dilute specific and non-specific peptides to 200 mg/ml in PBT. In a microtiter plate, mix equal volumes of the peptide dilutions and the MAb supe. Add an equal volume of PBT and MAb as the control. Incubate 1 hour at 37
Preparation Of Peptide-KLH Conjugates For Immunization
have a tendency to get lost after synthesis.(1) Make up 5 mM Ellman's reagent (dithio-bis-2-nitrobenzoic acid) in 0.1M NaPi pH 7.2.(2) Weigh out about 1 mg of peptide into a tared tube.(3) Add 0.5 ml reagent. It should go bright yellow.(4) Dilute the mixture 1/50
,with the development of ESI-MS and MALDI-MS.Peptide mass fingerprinting uses the peptide masses obtained by digestion to search the protein and DNA databases,to find proteins that show a similar theoretical digest pattern (see eg .,Shevchenko et al.1996a and b
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