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文献和实验of plates (above the -20 freezers) Yeast growth medium generally consists of three parts: a nitrogen base (supplied by SD), a sugar source (supplied by glucose) and necessary amino acids (supplied by CSM). These three components are what you''re going
Protein extraction from whole tissues for IEF
Extraction Medium (100 ml) - filter sterilize and store at 4 C 0.1 M Trizma base pH 8.8 1.21 g 10 mM EDTA 2 mls of 0.5 M stock 0.9 M sucrose 30.8 g 0.4% B-mercaptoethanol add 40 ul to 10 ml of buffer just prior to use prepare Tissue
Procedures for In Vitro Production of Bovine Embryos
(0.9%) Prepare 0.9% saline (9 g NaCl per liter) in double distilled water and autoclave. Label as "Sterile 0.9% saline, date made" and store indefinitely at 4°C.Oocyte Collection Medium (OCM) 1. Dissolve TCM-199 powder (without phenol red, and without glutamine
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