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文献和实验Methods for the Detection of D-Amino-Acid Oxidase
on the activity of the samples. It was terminated by the addition of 1 ml of 10% trichloroacetic acid. In a blank, trichloroacetic acid was added to the reaction mixture before the enzyme reaction was started. The precipitate was removed by centrifugation (700 x g
Unlabeled carboxylic acid substrate (e.g., 1 mM hydrocinnamic acid) [γ‐33 P]ATP of known concentration and specific activity (e.g., 10 mM, 6.8 cpm/pmol
Polycations have been used as nonviral gene carriers because the polycations and DNAs form stable complexes in a noncovalent manner (1 –3 ). The polycations, e.g., poly(L-lysine) (PLL), are reported to be conjugated with several ligands
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