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文献和实验and applied voltage. Invitrogen offers a range of UltraPure™ agarose products to meet your nucleic acid electrophoresis needs. As with all Invitrogen UltraPure™ reagents, these products are made from the highest purity biochemicals for maximum reliability
First-Strand Synthesis System for RT-PCR (Invitrogen). 25 bp DNA ladder (Invitrogen). ABI Prism 7000 Sequence Detection System (Applied Biosystems). ABI Prism 7000
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
2.1. 3' RACE LaNe 1. 20 ng total RNA (see Note 1). 2. 10 mM dNTPs. 3. ThermoScript ® Reverse Transcriptase and accompanyingbuffers (Invitrogen) (see Note 2). 4. TTT3 primer: 5'-gene-specific sequence followed byTTTTTTTTTTTTTTTTV
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