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Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase

The E. coli JS200 strain carries a temperature-sensitive allele of DNA polymerase I that renders this strain conditional lethal. Growth under restrictive conditions is restored by small amounts of DNA polymerase activity. Even mutants

SNP Discovery by Direct DNA Sequencing

DNA sequencing, while relatively laborious, is the gold standard in mutation detection and single nucleotide polymorphism (SNP) discovery. The most widely used approach is direct DNA sequencing of polymerase chain reaction (PCR) products

A Method to Assay Inhibitors of DNA Polymerase IIIC Activity

, one of these, designated pol III, is responsible for DNA replication and growth of bacteria and, therefore, warrants consideration as a drug target. However, the pol III enzymes of Gram-positive and Gram-negative species are quite different, and the Gram-positive enzyme