Applied Genetics Laboratories特

RNA-biotin based pulldown assays for the detection of siRNA targeted genomic regions and siRNA directed histone modifications (PROT32)

with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™

In Vivo Imaging of Far-red Fluorescent Proteins after DNA Electrotransfer to Muscle Tissue

were controlled by spectrophotometry. Plasmids were finally dissolved in PBS at a concentration of 0.25 µg/µl unless otherwise specified. In vivo DNA electrotransfer    Twenty-microliter plasmid solution was injected i.m. along the fibers

Two-colour fluorescent in situ DNA hybridization on whole mount Drosophila embryos and larval imaginal discs

al , 2004). Fluorescent in situ DNA hybridization (FISH) allows to study the positioning of single copy genes into the nucleus, and is therefore widely applied to address the question of how is gene positioning regulated.