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文献和实验transfer vessels. Each day students will observe these transfer cultures with an inverted phase contrast microscope, and remove aliquots for cell counting with a hemacytometer. Simultaneously, they will check on the viability of the cells through a dye
Use of the Hemacytometer for the Determination of Cell Numbers
Counting cells by the use of a hemacytometer is a convenient and practical method of determining cell numbers in the case that the Coulter counter is out-of-order temporarily. (It is not that bad.) The hemacytometer consists of two chambers
) or similar containers Incubator at 37°C with a 5%/95% CO2 /O2 atmosphere and 100% relative humidity Phase-contrast microscope Material for cell count (cell counting chamber, coverslips, 0.05% Trypan blue solution) 实验步骤
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