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文献和实验Bisulfite modification kits and PCR-DNA Methylation, His
of about 400 bp for cloning and we also need to use the template for MSP. Has anyone found that DNA is more easily amplified from one of these kits compared to the other? Thanks for your help! -Kiwicloner- ----
Is there a PCR length limitation after Bisulfite treatme
PCR. Use high quality kits to extract DNA and the fragile problem following bisulfite modification shoudl be decreased and then it should be possible to amplified lagger fragments compared with published papers. Good luck. -cryobeer-
Bisulfite sequencing of very small samples
Introduction (based on Olek et al., 1996, Schoenherr et al., 2003) DNA methylation is a stable epigenetic mark, which can mediate gene silencing. Bisulfite sequencing allows for precise identification of methylated cytosines
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