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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Chromatin Immunoprecipitation (ChIP)
of high-salt wash buffer for ChIP to all samples and rotate for 10 min at room temperature. 22. Centrifuge the samples at 3000 rpm for 2 min at room temperature. 23. Carefully aspirate the supernatants and add 1 mL of high
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
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